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reverse second overlap extension pcr puast mcd8 gfp  (Addgene inc)


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    Addgene inc reverse second overlap extension pcr puast mcd8 gfp
    Reverse Second Overlap Extension Pcr Puast Mcd8 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/overlap+extension+pcr/mCD8-GFP+in+pUAST+(Plasmid+%2317746)/pmc12557536__pnas%2E2503166122%2Esapp-147-99-105
    Average 93 stars, based on 16 article reviews
    reverse second overlap extension pcr puast mcd8 gfp - by Bioz Stars, 2026-09
    93/100 stars

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    Construction of tandem gene duplications. ( A ) PCR fragment with a selectable marker (“R”) at the novel junction. The fragments typically carry 75 base genome homologies at the ends. ( B ) Recombination with a replicating chromosome creates a gene duplication which carries the selectable marker R (usually for gentamicin resistance).

    Journal: mBio

    Article Title: Short tandem gene duplications as potential agents of genetic memory

    doi: 10.1128/mbio.02011-25

    Figure Lengend Snippet: Construction of tandem gene duplications. ( A ) PCR fragment with a selectable marker (“R”) at the novel junction. The fragments typically carry 75 base genome homologies at the ends. ( B ) Recombination with a replicating chromosome creates a gene duplication which carries the selectable marker R (usually for gentamicin resistance).

    Article Snippet: A DNA fragment corresponding to the deletion was constructed by extension overlap PCR and introduced into plasmid pU1 ( ) by USER cloning (New England Biolabs).

    Techniques: Marker